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The fourth tab
is used to overlay iso-contour lines onto the images. A histogram of the pixel values is shown, and the value at which the iso-contour lines are drawn can be adjusted by entering a numerical value, or by dragging the marker highlighted in the graphics above. The selection on the right allows choosing among several colors for the contour lines.
Per default, the iso-contour lines are shown as long as the iso-contour tab is selected. If another tab is selected, they disappear. To make them appear permanently in the images the pin button 
can be fixed to 
.